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for immunohistochemistry

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Old 03-31-2009, 12:27 PM
drvet_anjan drvet_anjan is offline
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for immunohistochemistry

hi everyone i am a new kid on the block. i have a question regarding image analysis of immunohistochemistry images taken from a camera attached to a microscope. here i am having a problem in analysing two colours separately as both the colours are localized in a particular location on a cell surface. i am using Image pro plus - as a imaging software.
can we appreciate each colour separately by any software like adobe photshop...... plz help me i have no idea regarding computer softwares... i am just a scientist stuck in this situation.
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Old 03-31-2009, 01:50 PM
frate frate is offline
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Re: for immunohistochemistry

I'm not familiar with that application. But this is likely a good starting point.
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Old 03-31-2009, 02:41 PM
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0lBaldy 0lBaldy is offline
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Re: for immunohistochemistry

If you are working in Windows you might consider the "Color Deconvolution Plug-in" <click

It takes some getting used to but does excellent color separation type stuff... Look at some samples HERE

For a small donation it might help!
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Old 03-31-2009, 05:31 PM
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lurch lurch is offline
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Re: for immunohistochemistry

ImageJ was designed for analyses just like yours.It's free and works in both OS X and Windows. Don't ask me how to use it, though - I've barely scratched its surface.
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Old 04-04-2009, 04:52 PM
gina gina is offline
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Re: for immunohistochemistry


We have Image Pro plus too and it's a very powerful program that should be able to do this. I suppose the color deconvolution plug-in would work nicely too.

I have used paint shop pro to select different colors for measuring using the different color channels. You can change the image from RGB (red-green-blue colors) to CMYK(cyan-magenta-yellow-black colors) and get different information that way.
In Paint Shop pro you can export those different channels as different grayscale images, this should be possible in IP and PS

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Old 07-04-2012, 02:56 PM
4N6site 4N6site is offline
Join Date: Jul 2012
Posts: 46
Arrow Re: for immunohistochemistry [fixed broken link]

The mentioned color deconvolution plug-in is from Check out some examples here.
Previous posts contain outdated links, but the plug-in is available through
It has a special feature to save/load settings which is useful for immunohistochemistry applications.
You can also contact the site if you want to be absolutely sure you get the maximum out of the plug-in!
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