Hello,
It is a common problem in microphotography that the illumination of the microscope field is not completely uniform.
Question: would it be thinkable to take two photos, #1 of the empty field, #2 with the object and combine them in one psd as layers and substract #1 from #2 to get rid of the background?
Masking is out of the question due to the contorted character of the object and the unavoidable loss of edge detail in touching up the mask.
I hope I did not miss the obvious answer to this in Richard's book,wich I confess to be still digesting.
It is a common problem in microphotography that the illumination of the microscope field is not completely uniform.
Question: would it be thinkable to take two photos, #1 of the empty field, #2 with the object and combine them in one psd as layers and substract #1 from #2 to get rid of the background?
Masking is out of the question due to the contorted character of the object and the unavoidable loss of edge detail in touching up the mask.
I hope I did not miss the obvious answer to this in Richard's book,wich I confess to be still digesting.
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